Overview

  • Product nameAnti-SGLT1 antibody
    See all SGLT1 primary antibodies
  • Description
    Rabbit polyclonal to SGLT1
  • Tested applicationsSuitable for: WB, Dot blot, ELISA, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1

  • Positive control
    • rat jejunal enterocyte basolateral membranes, rat kidney cortical membranes SGLT1 protein (ab99447)
  • General notes

    A blocking peptide for this product is available as ab190911.

Properties

Applications

Our Abpromise guarantee covers the use of ab14686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 74 kDa (predicted molecular weight: 73 kDa).Can be blocked with SGLT1 peptide (ab190911).
Dot blot 1/4000.
ELISA 1/4000.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. See Abreview.

Target

  • FunctionActively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Tissue specificityExpressed mainly in intestine and kidney.
  • Involvement in diseaseCongenital glucose/galactose malabsorption
  • Sequence similaritiesBelongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Post-translational
    modifications
    N-glycosylation is not necessary for the cotransporter function.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • D22S675 antibody
    • High affinity sodium glucose cotransporter 1 antibody
    • High affinity sodium glucose cotransporter antibody
    • High affinity sodium-glucose cotransporter antibody
    • Human Na+/glucose cotransporter 1 antibody
    • Na(+)/glucose cotransporter 1 antibody
    • Na+/glucose cotransporter 1 antibody
    • NAGT antibody
    • SC5A1_HUMAN antibody
    • SGLT 1 antibody
    • SGLT1 antibody
    • SLC5A1 antibody
    • Sodium glucose cotransporter 1 antibody
    • Sodium/glucose cotransporter 1 antibody
    • Solute carrier family 5 (sodium/glucose cotransporter) member 1 antibody
    • Solute carrier family 5 member 1 antibody
    see all

Anti-SGLT1 antibody images

  • Anti-SGLT1 antibody (ab14686) at 1/500 dilution

    Predicted band size : 73 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of formalin fixed, paraffin embedded porcine duodenum tissue from fasted animals. SGLT1 is stained with ab14686 at 1/50 diluton.

References for Anti-SGLT1 antibody (ab14686)

This product has been referenced in:
  • Pessoa TD  et al. Functional Role of Glucose Metabolism, Osmotic Stress, and Sodium-Glucose Cotransporter Isoform-Mediated Transport on Na+/H+ Exchanger Isoform 3 Activity in the Renal Proximal Tubule. J Am Soc Nephrol N/A:N/A (2014). Rat . Read more (PubMed: 24652792) »
  • Sajja RK  et al. Impact of altered glycaemia on blood-brain barrier endothelium: an in vitro study using the hCMEC/D3 cell line. Fluids Barriers CNS 11:8 (2014). ICC/IF ; Human . Read more (PubMed: 24708805) »

See all 10 Publications for this product

Product Wall

Thank you for your enquiry. I am afraid that there is no further information regarding the positive control that what is already stated on the datasheet. "The positive control for Sglt1 was purified rat jujenal brush border membranes solobilize...

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Thank you for your inquiry. I can confirm that rat SGLT1 has a predicted  molecular weight of ca. 73kDa. Molecular weights on gel are always only a rough estimate since the gel and buffer conditions can influence the resulting molecular weight of a pro...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish. It is known that more than 10 types of glucose transport carrier proteins designated as Glut 1-13 facilitate glucose transport across t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6,0
Permeabilization No
Blocking step Peroxidase Blocking solution (Dako) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney cortex)
Specification Kidney cortex
Fixative Formalin
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step Dako CSA II kit reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 03 2008

Thank you for your patience. For ab14685 the immunogen used was a synthetic peptide, corresponding to amino acids 603-623 of Human SGLT1. For ab14686 the immunogen used was a synthetic peptide, corresponding to amino acids 402-422 of Human SGLT1. Pl...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in re...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"