Overview

  • Product name
  • Description
    Rabbit polyclonal to SGLT2
  • Specificity
    The antibody detects 74 kDa SGLT-2 from human, mouse, and rat origins. Reactivity to other species has not been determined. A ~55 kDa and a ~45 kDa band can also be detected, representing the SGLT-2 cleavage fragments.
  • Tested applications
    Suitable for: IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide near the N terminus of SGLT2 (Mouse).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21872923
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 73 kDa.

Target

  • Function
    Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
    Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Involvement in disease
    Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test.
  • Sequence similarities
    Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Low affinity sodium glucose cotransporter antibody
    • Low affinity sodium-glucose cotransporter antibody
    • Na(+) glucose cotransporter 2 antibody
    • Na(+)/glucose cotransporter 2 antibody
    • OTTHUMP00000163298 antibody
    • SC5A2_HUMAN antibody
    • SGLT 2 antibody
    • SLC5A 2 antibody
    • SLC5A2 antibody
    • Sodium glucose cotransporter 2 antibody
    • Sodium/glucose cotransporter 2 antibody
    • Solute carrier family 5 (sodium glucose cotransporter) member 2 antibody
    • Solute carrier family 5 (sodium/glucose transporter), member 2 antibody
    • Solute carrier family 5 member 2 antibody
    see all

Images

  • Immunohistochemical analysis of mouse kidney frozen section, labeling SGLT2 with ab37296. Tissue sections were fixed with acetone. Blocking was with 2% horse serum for 1 hour at room temperature. Incubation for 16 hours at 4°C with ab37296 diluted 1/100 in 2% horse serum in 1XPBS.

    See Abreview

  • All lanes : Anti-SGLT2 antibody (ab37296) at 4 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Mouse small intestine tissue lysate


    Predicted band size : 73 kDa

References

This product has been referenced in:
  • Ji W  et al. Effects of canagliflozin on weight loss in high-fat diet-induced obese mice. PLoS One 12:e0179960 (2017). WB ; Mouse . Read more (PubMed: 28665967) »
  • Walmsley SJ  et al. Proteomic profiling of the effect of metabolic acidosis on the apical membrane of the proximal convoluted tubule. Am J Physiol Renal Physiol 302:F1465-77 (2012). Read more (PubMed: 22357915) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
None
Permeabilization
No
Specification
Kidney
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Aug 05 2015


Yes, different amounts of total protein are loaded to ensure effective detection in different tissues/cells since the level of expression with respect to total protein is different. There is ˜25 ug for the Jurkat cells, ˜75-100 ug in the small int...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Fixative
Acetone
Permeabilization
No
Blocking step
horse serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Mrs. Domenica Mccarthy

Verified customer

Submitted Apr 08 2013

DISCOUNT CODE: ****
Expiration date: dd mm yyyy

I am very pleased to hear you would like to accept our offer and test ab37296 in IHC-P. This code will give you the value of ab37296 off your next order before the expiration date. To redeem ...

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Thank you for contacting us.

I confirm you should suggest to your customer to apply for a testing discount if they want to use ab37296 in IHC-P or IHC-Fr.

Please note that the testing discount slightly changed very recently. It is now...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vi...

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Thank you for contacting us with your question. I have emailed the quotation in a separate email, so please let me know if you did not receive it. The SGLT2 antibody ab37296 is suitable for mouse and rat, and has been tested in Western blot and immunoc...

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Thank you for your enquiry. Unfortunately, the immunogen information for this product is proprietary. I am sorry I can't be of more assistance in this occasion. Should you require any further information, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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