Anti-SGLT2 antibody (ab85626)
Key features and details
- Rabbit polyclonal to SGLT2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SGLT2 antibody
See all SGLT2 primary antibodies -
Description
Rabbit polyclonal to SGLT2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SGLT2 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101414) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (6) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Sodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. -
Involvement in disease
Defects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test. -
Sequence similarities
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 399680 Cow
- Entrez Gene: 492301 Dog
- Entrez Gene: 6524 Human
- Entrez Gene: 246787 Mouse
- Entrez Gene: 64522 Rat
- Omim: 182381 Human
- SwissProt: P31639 Human
- SwissProt: Q923I7 Mouse
see all -
Alternative names
- Low affinity sodium glucose cotransporter antibody
- Low affinity sodium-glucose cotransporter antibody
- Na(+) glucose cotransporter 2 antibody
see all
Images
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IHC image of SGLT2 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85626, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab85626 has been referenced in 28 publications.
- Takashima M et al. Low-dose sodium-glucose cotransporter 2 inhibitor ameliorates ischemic brain injury in mice through pericyte protection without glucose-lowering effects. Commun Biol 5:653 (2022). IHC-P ; Mouse . PubMed: 35780235
- Locatelli M et al. Empagliflozin protects glomerular endothelial cell architecture in experimental diabetes through the VEGF-A/caveolin-1/PV-1 signaling pathway. J Pathol 256:468-479 (2022). PubMed: 35000230
- Uehara-Watanabe N et al. Direct evidence of proximal tubular proliferation in early diabetic nephropathy. Sci Rep 12:778 (2022). PubMed: 35039597
- Barkas G et al. Targeted proteomic analysis dataset of archival core human kidney biopsies to investigate the biology of hypertensive nephropathy. Data Brief 40:107805 (2022). PubMed: 35071703
- Du J et al. The expression and survival significance of sodium glucose transporters in pancreatic cancer. BMC Cancer 22:116 (2022). PubMed: 35090421