Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SGLT2.
Our Abpromise guarantee covers the use of ab85626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 73 kDa. PubMed: 25268124|
|ICC/IF||Use at an assay dependent concentration. PubMed: 25268124|
Immunohistochemical analysis of rat kidney frozen sections, labeling SGLT2 with ab85626. Sections were paraformaldehyde fixed, permeabilzed in 0.1% Triton-X, and blocked with 1% BSA for 30 minutes at 20°C. Immunostaining with anti-SGLT2 diluted 1/200 (1% BSA and 1% normal donkey serum in PBS) for 16 hours at 4°C.