Overview

  • Product nameAnti-SGLT2 antibody
    See all SGLT2 primary antibodies
  • Description
    Rabbit polyclonal to SGLT2
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human SGLT2.

  • Positive control
    • This antibody gave a positive signal in Human Kidney Tissue (Immunohistochemistry).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 73 kDa. PubMed: 25268124
ICC/IF Use at an assay dependent concentration. PubMed: 25268124

Target

  • FunctionSodium-dependent glucose transporter. Has a Na(+) to glucose coupling ratio of 1:1.
    Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
  • Involvement in diseaseDefects in SLC5A2 are the cause of renal glucosuria (GLYS1) [MIM:233100]. GLYS1 is an autosomal recessive disorder characterized by a normal fasting serum glucose concentration and persistent isolated glucosuria, with a normal glucose tolerance test.
  • Sequence similaritiesBelongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Low affinity sodium glucose cotransporter antibody
    • Low affinity sodium-glucose cotransporter antibody
    • Na(+) glucose cotransporter 2 antibody
    • Na(+)/glucose cotransporter 2 antibody
    • OTTHUMP00000163298 antibody
    • SC5A2_HUMAN antibody
    • SGLT 2 antibody
    • SLC5A 2 antibody
    • SLC5A2 antibody
    • Sodium glucose cotransporter 2 antibody
    • Sodium/glucose cotransporter 2 antibody
    • Solute carrier family 5 (sodium glucose cotransporter) member 2 antibody
    • Solute carrier family 5 (sodium/glucose transporter), member 2 antibody
    • Solute carrier family 5 member 2 antibody
    see all

Anti-SGLT2 antibody images

  • IHC image of SGLT2 staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85626, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-SGLT2 antibody (ab85626)

This product has been referenced in:
  • Jiang M  et al. Sodium-Glucose Transporter-2 (SGLT2; SLC5A2) Enhances Cellular Uptake of Aminoglycosides. PLoS One 9:e108941 (2014). WB, ICC/IF ; Mouse . Read more (PubMed: 25268124) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer(pH6)
Permeabilization No
Specification Kidney
Blocking step (agent) for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Formaldehyde
Username

Miss. Hsiu-Yun Huang

Verified customer

Submitted Mar 04 2016

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Kidney)
Permeabilization Yes - 0.1% Triton-X
Specification Kidney
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 08 2015

We do provide the immunogen peptide for ab85626. Our SGLT2 peptide (ab101414) may be found at the following location: http://www.abcam.com/sglt2-peptide-ab101414.html

DISCOUNT CODE: *******
Expiration date: DD MM YYYY
I am very pleased to hear you would like to accept our offer and test ab85626 in Mouse. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please...

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Thank you very much for your interest in ab85625.
To our knowledge, ab85626 has not been tested in Mouse. Therefore, I can offer a discount off a future purchase if you buy ab85625 now, test it in Mouse and submit feedback to us in the form of an ...

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DISCOUNT CODE: *** Expiration date: April 20, 2012 This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab85626 in guinea pig or mouse and include this code in the “Additional C...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"