Overview

  • Product nameAnti-SGOL2 antibody
  • Description
    Rabbit polyclonal to SGOL2
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ESHSHSDQSS KTSLMSEMRN AQSIGRRWEK PSPSNVTERK KRGSSWESNN LSADTPCATV LDKQHISSPE LNCNNEINGH TNETNTEMQR N, corresponding to amino acids 235-325 of Human SGOL2.

  • Positive control
    • Human testis tissue; A431 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 145 kDa.

Target

  • FunctionCooperates with PPP2CA to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Has a crucial role in protecting REC8 at centromeres from cleavage by separase. During meiosis, protects centromeric cohesion complexes until metaphase II/anaphase II transition, preventing premature release of meiosis-specific REC8 cohesin complexes from anaphase I centromeres. Is thus essential for an accurate gametogenesis. May act by targeting PPP2CA to centromeres, thus leading to cohesin dephosphorylation (By similarity). Essential for recruiting KIF2C to the inner centromere and for correcting defective kinetochore attachments.
  • Sequence similaritiesBelongs to the shugoshin family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. During meiosis I, accumulates at centromeres during diplotene, and co-localizes differentially with the cohesin subunits RAD21 and REC8 at metaphase I centromeres. SGO2 and RAD21 change their relative distributions during telophase I when sister-kinetochore association is lost. During meiosis II, it shows a striking tension-dependent redistribution within centromeres throughout chromosome congression during prometaphase II, as it does during mitosis (By similarity). In Hela cells, localizes at centromeres throughout prophase until metaphase and disappears at anaphase. Centromeric localization requires the presence of BUB1 and AUKRB.
  • Information by UniProt
  • Database links
  • Alternative names
    • Sgo2 antibody
    • Sgol2 antibody
    • SGOL2_HUMAN antibody
    • Shugoshin-2 antibody
    • Shugoshin-like 2 antibody
    • Tripin antibody
    see all

Anti-SGOL2 antibody images

  • Anti-SGOL2 antibody (ab122258) + HEL human cell line lysate

    Predicted band size : 145 kDa
  • ab122258, at 1/100, staining SGOL2 in paraffin embedded Human testis tissue by Immunohistochemistry.
  • ab122258, at 4 µg/ml, staining SGOL2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green stain).

References for Anti-SGOL2 antibody (ab122258)

ab122258 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"