Overview

  • Product nameAnti-SGSH antibody
    See all SGSH primary antibodies
  • Description
    Mouse polyclonal to SGSH
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    SGSH (NP_000190.1, 1 a.a. ~ 502 a.a) full-length human protein.

  • Positive control
    • SGSH transfected 293T cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab72617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.

Target

  • Involvement in diseaseDefects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A) [MIM:252900]; also known as Sanfilippo syndrome A. MPS3A is a severe form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life. MPS3A is characterized by earlier onset, rapid progression of symptoms and shorter survival.
  • Sequence similaritiesBelongs to the sulfatase family.
  • Post-translational
    modifications
    The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.
  • Cellular localizationLysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heparan sulfate sulfatase antibody
    • Heparan sulphate sulphatase antibody
    • HSS antibody
    • MPS 3A antibody
    • MPS3 A antibody
    • MPS3A antibody
    • Mucopolysaccharidosis type IIIA antibody
    • N sulfoglucosamine sulfohydrolase (sulfamidase) antibody
    • N sulfoglucosamine sulfohydrolase antibody
    • N-sulphoglucosamine sulphohydrolase antibody
    • SFMD antibody
    • SGSH antibody
    • SPHM_HUMAN antibody
    • Sulfoglucosamine sulfamidase antibody
    • Sulphamidase antibody
    • Sulphoglucosamine sulphamidase antibody
    see all

Anti-SGSH antibody images

  • All lanes : Anti-SGSH antibody (ab72617) at 1/500 dilution

    Lane 1 : SGSH transfected 293T cell line
    Lane 2 : Non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 57 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)

References for Anti-SGSH antibody (ab72617)

ab72617 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. Unfortunately, the details of the transfection are considered proprietary and I am unable to provide a protocol for the preparation of the cell line. We may be able to add the transfected lysate to our catalog, so please le...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"