Overview

  • Product nameAnti-SGT1 antibody
    See all SGT1 primary antibodies
  • Description
    Rabbit polyclonal to SGT1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (PAHMFGVTKF GDNIEDEWFI VYVIKQITKE FPELVARIED NDGEFLLIEA) of human SGT1 (NP_009196).

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

Anti-SGT1 antibody images

  • Anti-SGT1 antibody (ab82956) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-SGT1 antibody (ab82956)

ab82956 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH-3T3)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification NIH-3T3
Blocking step Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Dec 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"