Overview

  • Product name
  • Description
    Rabbit polyclonal to SGT1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 72-121 (PAHMFGVTKF GDNIEDEWFI VYVIKQITKE FPELVARIED NDGEFLLIEA) of human SGT1 (NP_009196).

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab82956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

Target

Anti-SGT1 antibody images

  • Anti-SGT1 antibody (ab82956) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-SGT1 antibody (ab82956)

ab82956 has not yet been referenced specifically in any publications.

Product Wall

Filter by Application

Filter by Species

Filter by Ratings

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH-3T3)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NIH-3T3
Blocking step
Milk as blocking agent for 6 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Dec 10 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up