Overview

  • Product name
  • Description
    Chicken polyclonal to SGTA
  • Host species
    Chicken
  • Specificity
    This antibody is specific for SGTA.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to SGTA aa 61-313 (C terminal).

Properties

Applications

Our Abpromise guarantee covers the use of ab37706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF 1/100.

Target

  • Function
    Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the SGT family.
    Contains 3 TPR repeats.
  • Domain
    The second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha SGT antibody
    • Alpha-SGT antibody
    • AlphaSGT antibody
    • hSGT antibody
    • SGT antibody
    • Sgt1 antibody
    • SGTA antibody
    • SGTA_HUMAN antibody
    • Small glutamine rich protein with tetratricopeptide repeats 1 antibody
    • Small glutamine rich tetratricopeptide repeat containing protein alpha antibody
    • Small glutamine rich tetratricopeptide repeat TPR containing alpha antibody
    • Small glutamine rich tetratricopeptide repeat-containing protein antibody
    • Small glutamine-rich tetratricopeptide repeat-containing protein alpha antibody
    • Stg antibody
    • UBP antibody
    • Vpu binding protein antibody
    • Vpu-binding protein antibody
    see all

Images

  • Anti-SGTA antibody (ab37706) at 1/2000 dilution + E. coli-derived fusion protein at 0.5 µg

    Secondary
    Goat anti-IgY-HRP. at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)



    A fusion protein with 6xhis-NusA, which adds 470 amino acids (around 55-60 kDa to the protein) was used in this western blot. The predicted SGTA protein is around 34 kDa. Therefore, the higher than expected band size was obtained.

References

This product has been referenced in:
  • Andreyeva A  et al. CHL1 is a selective organizer of the presynaptic machinery chaperoning the SNARE complex. PLoS One 5:e12018 (2010). WB ; Mouse . Read more (PubMed: 20711454) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (co-culture of hippocampal neurons and astrocytes)
Specification
co-culture of hippocampal neurons and astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton X-100 for 5 min
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 15 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up