Overview

  • Product nameAnti-SGTA antibody
    See all SGTA primary antibodies
  • Description
    Mouse monoclonal to SGTA
  • Tested applicationsSuitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: AFGVTVEDSD LALPQTLPEI FEAAATGKEM PQDLRSPART PPSEEDSAEA ERLKTEGNEQ MKVENFEAAV HFYGKAIELN PA, corresponding to amino acids 42-124 of Human SGTA

Properties

Applications

Our Abpromise guarantee covers the use of ab55689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.01-0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionCo-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the SGT family.
    Contains 3 TPR repeats.
  • DomainThe second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha SGT antibody
    • Alpha-SGT antibody
    • AlphaSGT antibody
    • hSGT antibody
    • SGT antibody
    • Sgt1 antibody
    • SGTA antibody
    • SGTA_HUMAN antibody
    • Small glutamine rich protein with tetratricopeptide repeats 1 antibody
    • Small glutamine rich tetratricopeptide repeat containing protein alpha antibody
    • Small glutamine rich tetratricopeptide repeat TPR containing alpha antibody
    • Small glutamine rich tetratricopeptide repeat-containing protein antibody
    • Small glutamine-rich tetratricopeptide repeat-containing protein alpha antibody
    • Stg antibody
    • UBP antibody
    • Vpu binding protein antibody
    • Vpu-binding protein antibody
    see all

Anti-SGTA antibody images

  • Overlay histogram showing HeLa cells stained with ab55689 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55689, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.


  • Predicted band size : 34 kDa
    SGTA antibody (ab55689) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab55689 at 10 ug/ml staining SGTA in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-SGTA antibody (ab55689)

ab55689 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"