Anti-SH2B1/PSM antibody (ab228828)
Key features and details
- Rabbit polyclonal to SH2B1/PSM
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SH2B1/PSM antibody
See all SH2B1/PSM primary antibodies -
Description
Rabbit polyclonal to SH2B1/PSM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment within Human SH2B1/PSM (internal sequence). The exact sequence is proprietary.
Database link: Q9NRF2 -
Positive control
- WB: C2C12, PC-12 and Rat2 whole cell extracts; Mouse and rat brain tissue extracts. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 79 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 79 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase (JAK) and receptor tyrosine kinases, including the receptors of insulin (INS), insulin-like growth factor I (IGF1), nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), platelet-derived growth factor (PDGF) and fibroblast growth factors (FGFs). In growth hormone (GH) signaling, autophosphorylated ('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin (LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis. -
Tissue specificity
Widely expressed with highest levels in skeletal muscle and ovary. -
Sequence similarities
Belongs to the SH2B adapter family.
Contains 1 PH domain.
Contains 1 SH2 domain. -
Post-translational
modificationsPhosphorylated on tyrosine residues in response to receptor kinase stimulation. Phosphorylated by RET. -
Cellular localization
Cytoplasm. Membrane. Nucleus. Shuttles between the nucleus and the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 506066 Cow
- Entrez Gene: 25970 Human
- Entrez Gene: 20399 Mouse
- Entrez Gene: 89817 Rat
- Omim: 608937 Human
- SwissProt: Q9NRF2 Human
- SwissProt: Q91ZM2 Mouse
- SwissProt: Q62985 Rat
see all -
Alternative names
- DKFZp547G1110 antibody
- FLJ30542 antibody
- KIAA1299 antibody
see all
Images
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All lanes : Anti-SH2B1/PSM antibody (ab228828) at 1/2000 dilution
Lane 1 : Non-transfected C2C12 (mouse myoblast cell line) whole cell extract
Lane 2 : C2C12 (mouse myoblast cell line) transfected with SH2B1/PSM shRNA, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa10% SDS-PAGE gel.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for SH2B1/PSM (green) using ab228828 at 1/500 dilution in ICC/IF.
Alpha-tubulin filaments were labeled with an anti-Alpha-tubulin antibody at 1/2000 dilution (red).
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All lanes : Anti-SH2B1/PSM antibody (ab228828) at 1/2000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-SH2B1/PSM antibody (ab228828) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab228828 has been referenced in 1 publication.
- Liu J et al. BBOX1-AS1 contributes to colorectal cancer progression by sponging hsa-miR-361-3p and targeting SH2B1. FEBS Open Bio N/A:N/A (2020). PubMed: 31984680