Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)

Overview

  • Product nameAnti-SH2D1A/SAP antibody [EPR3168]
    See all SH2D1A/SAP primary antibodies
  • Description
    Rabbit monoclonal [EPR3168] to SH2D1A/SAP
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SH2D1A/SAP aa 1-100 (internal sequence). The exact sequence is proprietary.
    Database link: O60880

  • Positive control
    • WB: Molt-4, Jurkat, PC-12 and K562 cell lysates and mouse brain tissue lysate.
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR3168
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionInhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.
    • Tissue specificityExpressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis.
    • Involvement in diseaseDefects in SH2D1A are a cause of lymphoproliferative syndrome X-linked type 1 (XLP1) [MIM:308240]; also known as X-linked lymphoproliferative disease (XLPD) or Duncan disease. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
    • Sequence similaritiesContains 1 SH2 domain.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DSHP antibody
      • Duncan disease SH2 protein antibody
      • Duncan disease SH2-protein antibody
      • EBVS antibody
      • IMD5 antibody
      • LYP antibody
      • MTCP1 antibody
      • SAP antibody
      • SAP/SH2D1A antibody
      • SH2 domain containing 1A antibody
      • SH2 domain protein 1A antibody
      • SH2 domain-containing protein 1A antibody
      • SH21A_HUMAN antibody
      • SH2D1A antibody
      • Signaling lymphocyte activation molecule associated protein antibody
      • Signaling lymphocytic activation molecule-associated protein antibody
      • SLAM associated protein antibody
      • SLAM associated protein/SH2 domain protein 1A antibody
      • SLAM-associated protein antibody
      • T cell signal transduction molecule SAP antibody
      • T-cell signal transduction molecule SAP antibody
      • XLP antibody
      • XLPD antibody
      see all

    Anti-SH2D1A/SAP antibody [EPR3168] images

    • All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)

      Lane 1 : PC-12 whole cell lysate
      Lane 2 : Mouse brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 14 kDa
      Observed band size : 16 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)

      Lane 1 : Molt-4 whole cell lysate
      Lane 2 : Jurkat whole cell lysate
      Lane 3 : K562 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

      Predicted band size : 14 kDa
      Observed band size : 16 kDa (why is the actual band size different from the predicted?)

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/1000 dilution (unpurified)

      Lane 1 : MOLT-4 cell line lysate
      Lane 2 : Jurkat cell line lysate
      Lane 3 : K562 cell line lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa

    References for Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)

    ab109120 has not yet been referenced specifically in any publications.

    Product Wall

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    Thank you for your inquiry.
    The concentration for ab109120, lot GR61496-3, is 0.781 mg/mL.
    I hope this information helps. Please contact us with any other questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"