The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionInhibits estrogen-induced cell proliferation by competing with PLCG for binding to ESR1, blocking the effect of estrogen on PLCG and repressing estrogen-induced proliferation. May play a role in T-cell development and function.
Tissue specificityUbiquitously expressed. Aberrantly expressed in some cancers.
Sequence similaritiesContains 1 SH2 domain.
Cellular localizationCytoplasm. Located at podocyte foot processes.