Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling SH3BGRL with ab80865 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - SH3BGRL. Right - Merge.
Western blot - SH3BGRL antibody (ab80865)
Anti-SH3BGRL antibody (ab80865) at 1 µg/ml + MCF7 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000. Developed using the ECL technique