Overview

  • Product name
  • Description
    Rabbit polyclonal to SH3BGRL3
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: SEVTRILDGK RIQYQLVDIS QDNALRDEMR ALAGNPKATP PQIVNGDQYC GDYELFVEAV EQNTLQEFLK LA, corresponding to amino acids 22-93 of Human SH3BGRL3

  • Positive control
    • Human smooth muscle tissue; RT4 cell lysates and Human liver and tonsil tissue lysates; U2OS cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixed/permeabilized with PFA/Triton X-100

Target

  • Function
    Could act as a modulator of glutaredoxin biological activity.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the SH3BGR family.
    Contains 1 glutaredoxin domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • epididymis secretory protein Li 297 antibody
    • HEL-S-297 antibody
    • SH3 domain binding glutamic acid rich protein like 3 antibody
    • SH3 domain-binding glutamic acid-rich-like protein 3 antibody
    • SH3 domain-binding protein 1 antibody
    • Sh3bgrl3 antibody
    • SH3BGRL3 like protein antibody
    • SH3BP-1 antibody
    • SH3L3_HUMAN antibody
    • TIP B1 antibody
    • TIP-B1 antibody
    • TNF inhibitory protein antibody
    see all

Images

  • ab122138, at 1/600 dilution, staining SH3BGRL3 in Human smooth muscle tissue by Immunohistochemistry using paraffin-embedded tissue.
  • All lanes : Anti-SH3BGRL3 antibody (ab122138) at 1/250 dilution

    Lane 1 : RT4 cell lysates
    Lane 2 : U251MG cell lysates
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • ab122138 at 4 µg/ml staining SH3BGRL3 in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence. Antibody staining is shown in green

References

ab122138 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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