Overview

  • Product name
  • Description
    Goat polyclonal to SH3BP1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: PQPRPRSLASETN, corresponding to C terminal amino acids 689-701 of Human SH3BP1.

  • Positive control
    • Human kidney, brain and lung tissue lysate. Human heart and kidney tissue sections.
  • General notes


    Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to c-Abl proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac (By similarity).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to c-Abl proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac (By similarity).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).Can be blocked with Human SH3BP1 peptide (ab23182).
IHC-P Use a concentration of 0.3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval with citrate buffer pH 6 via the microwave method before commencing with IHC staining protocol.

Target

  • Function
    Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac.
  • Sequence similarities
    Contains 1 BAR domain.
    Contains 1 Rho-GAP domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3BP 1 antibody
    • 3BP-1 antibody
    • 3BP1_HUMAN antibody
    • ARHGAP43 antibody
    • dJ37E16 antibody
    • FLJ21318 antibody
    • OTTHUMP00000028981 antibody
    • SH3 domain binding protein 1 antibody
    • SH3 domain-binding protein 1 antibody
    • Sh3bp1 antibody
    see all

Images



  • Predicted band size : 71 kDa
    ab10103 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by Western blot using chemiluminescence. ab10103 staining (1µg/ml) of human brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
  • ab10103 (0.3µg/ml) staining SH3BP1 in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in a glomerulus of the kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

References

ab10103 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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