The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.
Expressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas.
Involvement in disease
Defects in SH3BP2 are the cause of cherubism (CRBM) [MIM:118400]. CRBM is an autosomal dominant inherited syndrome characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.
Detection of Human SH3BP2 by Immunoprecipitation of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab117833 at 6 µg/mg of lysate. Subsequent WB detection was done using an other anti Human SH3BP2 antibody.
Detection: Chemiluminescence with an exposure time of 10