Overview

  • Product nameAnti-SH3BP2 antibody
    See all SH3BP2 primary antibodies
  • Description
    Rabbit polyclonal to SH3BP2
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 432-481 (RQPSQADTGG DDSDEDYEKV PLPNSVFVNT TESCEVERLF KATSPRGEPQ) of Human SH3BP2 (NP_003014)

  • Positive control
    • 721_B cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab84297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionBinds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism.
  • Tissue specificityExpressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in SH3BP2 are the cause of cherubism (CRBM) [MIM:118400]. CRBM is an autosomal dominant inherited syndrome characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling.
  • Sequence similaritiesContains 1 PH domain.
    Contains 1 SH2 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3BP-2 antibody
    • 3BP2 antibody
    • 3BP2_HUMAN antibody
    • Abl SH3 binding protein 2 antibody
    • Cherubism antibody
    • CRBM antibody
    • CRPM antibody
    • FLJ42079 antibody
    • FLJ54978 antibody
    • RES4-23 antibody
    • SH3 domain binding protein 2 antibody
    • SH3 domain-binding protein 2 antibody
    • Sh3bp2 antibody
    • TNFAIP3 interacting protein 2 antibody
    see all

Anti-SH3BP2 antibody images

  • Anti-SH3BP2 antibody (ab84297) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab84297 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84297, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab84297 at 5 µg/ml staining SH3BP2 in Human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab84297 at 5 µg/ml staining SH3BP2 in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-SH3BP2 antibody (ab84297)

ab84297 has not yet been referenced specifically in any publications.

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