Overview

  • Product nameAnti-SH3BP4 antibody [2992C1a]
    See all SH3BP4 primary antibodies
  • Description
    Mouse monoclonal [2992C1a] to SH3BP4
  • Tested applicationsSuitable for: Dot blot, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment with a tag corresponding to N terminal amino acids 28-217 of Human SH3BP4.

  • Positive control
    • Immunising recombinant Human SH3BP4 protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab84853 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
  • ClonalityMonoclonal
  • Clone number2992C1a
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 107 kDa.

Target

  • FunctionFunctions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis.
  • Tissue specificityExpressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).
  • Sequence similaritiesContains 1 SH3 domain.
  • DomainThe SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity.
  • Post-translational
    modifications
    Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2.
  • Cellular localizationMembrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Nucleus. Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOG25 antibody
    • EH binding protein antibody
    • EH binding protein 10 antibody
    • EH-binding protein 10 antibody
    • EHB10 antibody
    • SH3 domain binding protein 4 antibody
    • SH3 domain-binding protein 4 antibody
    • SH3B4_HUMAN antibody
    • sh3bp4 antibody
    • Transferrin receptor trafficking protein antibody
    • Transferrin receptor-trafficking protein antibody
    • TTP antibody
    see all

Anti-SH3BP4 antibody [2992C1a] images

References for Anti-SH3BP4 antibody [2992C1a] (ab84853)

ab84853 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"