Purification notesab84853 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use at an assay dependent dilution. Predicted molecular weight: 107 kDa.
FunctionFunctions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis.
Tissue specificityExpressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).
Sequence similaritiesContains 1 SH3 domain.
DomainThe SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity.
Post-translational modificationsPhosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2.
Cellular localizationMembrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Nucleus. Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.