Overview

  • Product name
    Anti-SH3BP4 antibody
  • Description
    Rabbit polyclonal to SH3BP4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to 18 amino acids near the C terminal end of Human SH3BP4 (NP_055336).

  • Positive control
    • Rat lung tissue lysate. Human lung tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (stable for up to 12 months).
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 107 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Functions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis.
  • Tissue specificity
    Expressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).
  • Sequence similarities
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity.
  • Post-translational
    modifications
    Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2.
  • Cellular localization
    Membrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Nucleus. Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BOG25 antibody
    • EH binding protein antibody
    • EH binding protein 10 antibody
    • EH-binding protein 10 antibody
    • EHB10 antibody
    • SH3 domain binding protein 4 antibody
    • SH3 domain-binding protein 4 antibody
    • SH3B4_HUMAN antibody
    • sh3bp4 antibody
    • Transferrin receptor trafficking protein antibody
    • Transferrin receptor-trafficking protein antibody
    • TTP antibody
    see all

Images

  • Lane 1 : Anti-SH3BP4 antibody (ab106609) at 1 µg/ml
    Lane 2 : Anti-SH3BP4 antibody (ab106609) at 2 µg/ml

    Lane 1 : Rat lung tissue lysate
    Lane 2 : Rat lung tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 107 kDa
  • ab106609, at 5µg/ml, staining SH3BP4 in paraffin embedded Human lung tissue by Immunohistochemistry.

References

ab106609 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam regarding our SH3BP4 antibodies.


Regarding ab106609, this antibody is specific for isoform 1.


The immunogen of antibody ab57005 shares 97% identity with the mouse and rat sequences, thus it is ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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