Overview

  • Product nameAnti-SH3BP4 antibody
    See all SH3BP4 primary antibodies
  • Description
    Mouse monoclonal to SH3BP4
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-110 of Human SH3BP4

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab57005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionFunctions in transferrin receptor internalization at the plasma membrane through a cargo-specific control of clathrin-mediated endocytosis.
    • Tissue specificityExpressed in all tissues tested with higher expression in pancreas. Expressed by retinal pigment epithelial cells (at protein level).
    • Sequence similaritiesContains 1 SH3 domain.
    • DomainThe SH3 domain mediates localization to the clathrin-coated pits and vesicles. The SH3 domain mediates interaction with DNM2 and the cytoplasmic part of TFRC with a lower affinity.
    • Post-translational
      modifications
      Phosphorylated upon EGF stimulation. Phosphorylation prevents interaction with DNM2.
    • Cellular localizationMembrane > clathrin-coated pit. Cytoplasmic vesicle > clathrin-coated vesicle. Nucleus. Specifically associated with transferrin receptor-containing clathrin-coated pits and clathrin-coated vesicles. May also localize to the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BOG25 antibody
      • EH binding protein antibody
      • EH binding protein 10 antibody
      • EH-binding protein 10 antibody
      • EHB10 antibody
      • SH3 domain binding protein 4 antibody
      • SH3 domain-binding protein 4 antibody
      • SH3B4_HUMAN antibody
      • sh3bp4 antibody
      • Transferrin receptor trafficking protein antibody
      • Transferrin receptor-trafficking protein antibody
      • TTP antibody
      see all

    Anti-SH3BP4 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57005 SH3BP4 antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa

    References for Anti-SH3BP4 antibody (ab57005)

    ab57005 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting Abcam regarding our SH3BP4 antibodies.


    Regarding ab106609, this antibody is specific for isoform 1.


    The immunogen of antibody ab57005 shares 97% identity with the mouse and rat sequences, thus it is ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"