Overview

  • Product nameAnti-SH3GL2 antibody
    See all SH3GL2 primary antibodies
  • Description
    Mouse monoclonal to SH3GL2
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: SRAKLSMINT MSKIRGQEKG PGYPQAEALL AEAMLKFGRE LGDDCNFGPA LGEVGEAMRE L, corresponding to amino acids 64-125 of Human SH3GL2

  • Positive control
    • IF: HepG2 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab55702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionImplicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.
  • Tissue specificityBrain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
  • Sequence similaritiesBelongs to the endophilin family.
    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.
  • Cellular localizationCytoplasm. Membrane. Concentrated in presynaptic nerve terminals in neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA335L15.1 (SH3 domain GRB2 like 2) antibody
    • CNSA2 antibody
    • EEN B1 antibody
    • EEN-B1 antibody
    • Endophilin 1 antibody
    • Endophilin A1 antibody
    • Endophilin A1 BAR domain antibody
    • Endophilin-1 antibody
    • Endophilin-A1 antibody
    • FLJ20276 antibody
    • FLJ25015 antibody
    • OTTHUMP00000021084 antibody
    • SH3 domain containing GRB2 like protein 2 antibody
    • SH3 domain GRB2 like 2 antibody
    • SH3 domain protein 2A antibody
    • SH3 domain-containing GRB2-like protein 2 antibody
    • SH3D2A antibody
    • SH3G2_HUMAN antibody
    • Sh3gl2 antibody
    • Sh3p4 antibody
    • SH3PA antibody
    see all

Anti-SH3GL2 antibody images

  • SH3GL2 antibody (ab55702) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebral cortex.


  • Predicted band size : 40 kDa
    SH3GL2 antibody (ab55702) at 1ug/lane + PC-12 cell lysate at 25ug/lane.
  • Overlay histogram showing SH-SY5Y cells stained with ab55702 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55702, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ICC/IF image of ab55702 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55702, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SH3GL2 antibody (ab55702)

ab55702 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Mouse Cell (11DIV primary neurons)
Specification 11DIV primary neurons
Permeabilization Yes - 0.05% Triton x100 in TBS or PBS
Fixative Formaldehyde
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Ms. Eva Borger

Verified customer

Submitted Jun 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"