The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa.
Use a concentration of 3 µg/ml.
Use a concentration of 10 µg/ml.
Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionImplicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.
Tissue specificityBrain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
Sequence similaritiesBelongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain.
DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.
Cellular localizationCytoplasm. Membrane. Concentrated in presynaptic nerve terminals in neurons.
SH3 domain containing GRB2 like protein 2 antibody
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SH3 domain-containing GRB2-like protein 2 antibody
Anti-SH3GL2 antibody images
IHC-P - SH3GL2 antibody (ab55702)
SH3GL2 antibody (ab55702) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human cerebral cortex.
Western blot - SH3GL2 antibody (ab55702)
Predicted band size : 40 kDa SH3GL2 antibody (ab55702) at 1ug/lane + PC-12 cell lysate at 25ug/lane.
Flow Cytometry-Anti-SH3GL2 antibody(ab55702)
Overlay histogram showing SH-SY5Y cells stained with ab55702 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55702, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ICC/IF image of ab55702 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55702, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-SH3GL2 antibody (ab55702)
This product has been referenced in:
Poudel KR et al. A time course of orchestrated endophilin action in sensing, bending, and stabilizing curved membranes. Mol Biol Cell27:2119-32 (2016).
Read more (PubMed: 27170174) »