The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionImplicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.
Tissue specificityBrain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
Sequence similaritiesBelongs to the endophilin family. Contains 1 BAR domain. Contains 1 SH3 domain.
DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.
Cellular localizationCytoplasm. Membrane. Concentrated in presynaptic nerve terminals in neurons.