Recombinant
RabMAb

Anti-SH3GL2 antibody [EPR10214] (ab169762)

Overview

  • Product name
    Anti-SH3GL2 antibody [EPR10214]
    See all SH3GL2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10214] to SH3GL2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide within Human SH3GL2. The exact sequence is proprietary.
    Database link: Q99962

  • Positive control
    • Mouse brain, rat brain, Human cerebellum and Human fetal brain lysates; Human brain and Human glioma tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P 1/250 - 1/500.
IP 1/10 - 1/100.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.
  • Tissue specificity
    Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum.
  • Sequence similarities
    Belongs to the endophilin family.
    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • Domain
    An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes. The BAR domain dimer forms a rigid crescent shaped bundle of helices with the pair of second amphipathic helices protruding towards the membrane-binding surface.
  • Cellular localization
    Cytoplasm. Membrane. Concentrated in presynaptic nerve terminals in neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA335L15.1 (SH3 domain GRB2 like 2) antibody
    • CNSA2 antibody
    • EEN B1 antibody
    • EEN-B1 antibody
    • Endophilin 1 antibody
    • Endophilin A1 antibody
    • Endophilin A1 BAR domain antibody
    • Endophilin-1 antibody
    • Endophilin-A1 antibody
    • FLJ20276 antibody
    • FLJ25015 antibody
    • OTTHUMP00000021084 antibody
    • SH3 domain containing GRB2 like protein 2 antibody
    • SH3 domain GRB2 like 2 antibody
    • SH3 domain protein 2A antibody
    • SH3 domain-containing GRB2-like protein 2 antibody
    • SH3D2A antibody
    • SH3G2_HUMAN antibody
    • Sh3gl2 antibody
    • Sh3p4 antibody
    • SH3PA antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling SH3GL2 with ab169762 at 1/250 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SH3GL2 with ab169762 at 1/250 dilution.

  • ab169762 showing +ve staining in Mouse brain tissue.

  • ab169762 showing -ve staining in Human uterus tissue.
  • ab169762 showing -ve staining in Human thyroid gland carcinoma tissue.
  • ab169762 showing -ve staining in Human skeletal muscle tissue.
  • Detection of SH3GL2  by Western Blot of Immunprecipitate.
    Human fetal brain lysate cell lysate  (lane 1)  or 1 X PBS negaative control (lane 2) immunoprecipitated using ab169762 at 1/10 dilution.



    All lanes : Anti-SH3GL2 antibody [EPR10214] (ab169762) at 1/1000 dilution

    Lane 1 : Anti-SH3GL2 antibody [EPR10214] (ab169762) at 1/10 dilution
    Lane 2 : 1 X PBS negative control

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG
  • All lanes : Anti-SH3GL2 antibody [EPR10214] (ab169762) at 1/1000 dilution

    Lane 1 : mouse brain lysate
    Lane 2 : rat brain lysate
    Lane 3 : Human cerebellum lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 40 kDa

  • ab169762 staining SH3GL2 in mouse primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 2% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.05% Triton X-100 + 0.05% Tween20 in PBS and blocked with 1% BSA for 30 minutes at 22°C. Samples were incubated with primary antibody (1/150 in PBS + 1% BSA) for 2 hour at 22°C. An Alexa Fluor® 594-conjugated goat anti-rabbit IgG polyclonal (1/1500) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Chiang CK  et al. The proteomic landscape of the suprachiasmatic nucleus clock reveals large-scale coordination of key biological processes. PLoS Genet 10:e1004695 (2014). WB ; Mouse . Read more (PubMed: 25330117) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Mouse Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Permeabilization
Yes - 0.05%Tx100 + 0.05% Tween20 in PBS
Fixative
Paraformaldehyde
Username

Ms. Eva Borger

Verified customer

Submitted Apr 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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