Overview

  • Product nameAnti-SH3MD2 antibody
    See all SH3MD2 primary antibodies
  • Description
    Rabbit polyclonal to SH3MD2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide from within residues: LKLLSGASTK RKPRVSPPAS PTLEVELGSA ELPLQGAVGP ELPPGGGHGR, corresponding to internal sequence amino acids 720-769 of Human SH3MD2 (NP_065921).

  • Positive control
    • DU145 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab90180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceSH3MD2 acts as a scaffold protein, contributes to Rac-induced signal transduction such as JNKs (MAPK8 and MAPK9) activation and induces apoptosis. Within a signaling complex, it probably recruits protein kinases such as MAP3K10 or MAP3K11 which are in turn activated leading to the sequential activation of MAP2K4, MAP2K7 and JNKs (MAPK8 and MAPK9). SH3MD2 may be involved in targeting of HIV-1 GAG and GAG-POL polyproteins to the plasma membrane. This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. There are two named isoforms.
  • Cellular localizationGolgi Apparatus. Lamellipodium. Perinuclear region of cytoplasm.
  • Database links
  • Alternative names
    • FLJ21602 antibody
    • KIAA1494 antibody
    • Plenty of SH3 domains antibody
    • Plenty of SH3s antibody
    • POSH antibody
    • Ring finger protein 142 antibody
    • RNF 142 antibody
    • RNF142 antibody
    • SH3 domain containing ring finger 1 antibody
    • SH3 domain containing RING finger protein 1 antibody
    • SH3 multiple domains 2 antibody
    • SH3RF1 antibody
    see all

Anti-SH3MD2 antibody images

  • Anti-SH3MD2 antibody (ab90180) at 1 µg/ml + DU145 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa

References for Anti-SH3MD2 antibody (ab90180)

ab90180 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"