Validated using a knockout cell line
Recombinant
RabMAb

Anti-SH3PX1 antibody [EPR14399] (ab181856)

Overview

  • Product name
    Anti-SH3PX1 antibody [EPR14399]
    See all SH3PX1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14399] to SH3PX1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SH3PX1 aa 50-200. The exact sequence is proprietary.
    Database link: Q9Y5X1

  • Positive control
    • HeLa and HCT-116 cells and cell lysates; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab181856 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 78 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/100.
Flow Cyt 1/10. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.

Target

  • Function
    May be involved in several stages of intracellular trafficking. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.
  • Sequence similarities
    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
    Contains 1 SH3 domain.
  • Domain
    The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol-4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates.
  • Post-translational
    modifications
    Ubiquitinated by ITCH.
    Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR.
  • Cellular localization
    Cytoplasmic vesicle membrane. Cell membrane. Cytoplasmic vesicle > clathrin-coated vesicle. Golgi apparatus > trans-Golgi network. Cell projection > ruffle. Cytoplasm. Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • MST 155 antibody
    • MST155 antibody
    • MSTP 155 antibody
    • MSTP155 antibody
    • OTTHUMP00000040083 antibody
    • Protein SDP 1 antibody
    • Protein SDP1 antibody
    • SDP 1 antibody
    • SDP1 antibody
    • SH3 and PX domain containing protein 3A antibody
    • SH3 and PX domain containing protein SH3PX1 antibody
    • SH3 and PX domain-containing protein 1 antibody
    • SH3 and PX domain-containing protein 3A antibody
    • SH3PX1 antibody
    • SH3PXD3A antibody
    • SNX 9 antibody
    • SNX9 antibody
    • SNX9_HUMAN antibody
    • Sorting nexin 9 antibody
    • Sorting nexin-9 antibody
    • WASP interactor protein antibody
    • Wiskott Aldrich syndrome protein (WASP) interactor protein antibody
    • Wiskott Aldrich syndrome protein interactor protein antibody
    • WISP antibody
    see all

Images



  • Predicted band size : 67 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SH3PX1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HCT116 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181856 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181856 was shown to specifically react with SH3PX1 when SH3PX1 knockout samples were used. Wild-type and SH3PX1 knockout samples were subjected to SDS-PAGE. ab181856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot analysis of HepG2 cell lysate immunoprecipitated using ab181856 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SH3PX1 with ab181856 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling SH3PX1 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling SH3PX1 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SH3PX1 with ab181856 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Anti-SH3PX1 antibody [EPR14399] (ab181856) at 1/10000 dilution + HCT-116 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 67 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)
  • Anti-SH3PX1 antibody [EPR14399] (ab181856) at 1/50000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 67 kDa
    Observed band size : 78 kDa (why is the actual band size different from the predicted?)

References

ab181856 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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