Overview

  • Product name
  • Description
    Rabbit polyclonal to SH3PXD2B
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 556-642 (PPGVILPMMP AKHIPPARDS RRPEPKPDKS RLFQLKNDMG LECGHKVLAK EVKKPNLRPI SKSKTDLPEE KPDATPQNPF LKSRPQV) of Human SH3PXD2B

  • Positive control
    • Human pancreas tissue; Human tonsil, RT-4 and U-251 MG cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122342 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

  • Function
    Adapter protein involved in invadopodia and podosome formation and extracellular matrix degradation. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1-or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. Plays a role in mitotic clonal expansion during the immediate early stage of adipocyte differentiation.
  • Tissue specificity
    Expressed in fibroblasts.
  • Involvement in disease
    Defects in SH3PXD2B are the cause of Frank-Ter Haar syndrome (FTHS) [MIM:249420]. It is a syndrome characterized by brachycephaly, wide fontanels, prominent forehead, hypertelorism, prominent eyes, macrocornea with or without glaucoma, full cheeks, small chin, bowing of the long bones and flexion deformity of the fingers.
  • Sequence similarities
    Belongs to the SH3PXD2 family.
    Contains 1 PX (phox homology) domain.
    Contains 4 SH3 domains.
  • Domain
    The PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-biphosphate (PtdIns(5)P), and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain.
  • Post-translational
    modifications
    Phosphorylated in SRC-transformed cells.
  • Cellular localization
    Cytoplasm. Cell projection > podosome. Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adapter protein HOFI antibody
    • Factor for adipocyte differentiation 49 antibody
    • FAD49 antibody
    • FLJ20831 antibody
    • FTHS antibody
    • KIAA1295 antibody
    • SH3 and PX domain-containing protein 2B antibody
    • SH3PXD2B antibody
    • SPD2B_HUMAN antibody
    • TKS4 antibody
    • Tyrosine kinase substrate with four SH3 domains antibody
    see all

Images

  • ab122342, at a 1/200 dilution, staining SH3PXD2B in paraffin-embedded Human pancreas tissue by immunohistochemistry.
  • All lanes : Anti-SH3PXD2B antibody (ab122342) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References

ab122342 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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