Overview

  • Product name
  • Description
    Rabbit polyclonal to SH3PXD2B
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 300-350 of Human SH3PXD2B using the numbering given in entry NP_001017995.1.

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab123503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Adapter protein involved in invadopodia and podosome formation and extracellular matrix degradation. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1-or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. Plays a role in mitotic clonal expansion during the immediate early stage of adipocyte differentiation.
    • Tissue specificity
      Expressed in fibroblasts.
    • Involvement in disease
      Defects in SH3PXD2B are the cause of Frank-Ter Haar syndrome (FTHS) [MIM:249420]. It is a syndrome characterized by brachycephaly, wide fontanels, prominent forehead, hypertelorism, prominent eyes, macrocornea with or without glaucoma, full cheeks, small chin, bowing of the long bones and flexion deformity of the fingers.
    • Sequence similarities
      Belongs to the SH3PXD2 family.
      Contains 1 PX (phox homology) domain.
      Contains 4 SH3 domains.
    • Domain
      The PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-biphosphate (PtdIns(5)P), and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain.
    • Post-translational
      modifications
      Phosphorylated in SRC-transformed cells.
    • Cellular localization
      Cytoplasm. Cell projection > podosome. Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adapter protein HOFI antibody
      • Factor for adipocyte differentiation 49 antibody
      • FAD49 antibody
      • FLJ20831 antibody
      • FTHS antibody
      • KIAA1295 antibody
      • SH3 and PX domain-containing protein 2B antibody
      • SH3PXD2B antibody
      • SPD2B_HUMAN antibody
      • TKS4 antibody
      • Tyrosine kinase substrate with four SH3 domains antibody
      see all

    Images

    • Detection of Human SH3PXD2B by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123503 at 6 µg/mg lysate for IP
      Subsequent Western blot detection used an antibody which recognized a downstream epitope of SH3PXD2B.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab123503 has not yet been referenced specifically in any publications.

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