Overview

  • Product name
  • Description
    Rabbit polyclonal to SHANK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 2100 and 2161 of Human SHANK1 (Q9Y566)

  • Positive control
    • 293T whole cell lysate, SW480 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 225 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction.
  • Tissue specificity
    Expressed in brain particularly in the amygdala, hippocampus, substantia nigra and thalamus. Isoform 2 seems to be expressed ubiquitously.
  • Sequence similarities
    Belongs to the SHANK family.
    Contains 6 ANK repeats.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density of neuronal cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • GKAP/SAPAP interacting protein antibody
    • OTTHUMP00000174437 antibody
    • SH3 and multiple ankyrin repeat domains 1 antibody
    • SH3 and multiple ankyrin repeat domains protein 1 antibody
    • SH3/ankyrin domain gene 1 antibody
    • SHAN1_HUMAN antibody
    • SHANK 1 antibody
    • Shank1 antibody
    • Shank1a antibody
    • Somatostatin receptor interacting protein antibody
    • Somatostatin receptor-interacting protein antibody
    • SPANK 1 antibody
    • SPANK1 antibody
    • SSTR interacting protein antibody
    • SSTR-interacting protein antibody
    • SSTRIP antibody
    • Synamon antibody
    see all

Images

  • Anti-SHANK1 antibody (ab154224) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 225 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human J5 xenograft tissue labelling SW480 with ab154224 at a 1/1000 dilution.

References

ab154224 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154224.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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