Overview

  • Product name
    Anti-SHANK2 antibody [N23B/49]
    See all SHANK2 primary antibodies
  • Description
    Mouse monoclonal [N23B/49] to SHANK2
  • Host species
    Mouse
  • Specificity
    Recognizes SHANK1, SHANK2, SHANK3.
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to amino acids 84-309 (SH3/PDZ domains) of Rat SHANK2 (Q9QX74).

  • Positive control
    • Rat brain lysate
  • General notes

    The clone number has been updated from S23B-49 to N23B/49, both clone numbers name the same antibody clone.

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    N23B/49
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab94575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
ICC
ICC/IF
  • Application notes
    ICC: Use at a concentration of 0.1 - 1 µg/ml.
    ICC/IF: Use at a concentration of 1 - 10 µg/ml.
    IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
    IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
    WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 159 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
    • Tissue specificity
      Expressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level).
    • Sequence similarities
      Belongs to the SHANK family.
      Contains 1 PDZ (DHR) domain.
      Contains 1 SAM (sterile alpha motif) domain.
      Contains 1 SH3 domain.
    • Developmental stage
      Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum.
    • Domain
      The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR.
    • Cellular localization
      Apical cell membrane. Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, growth cone. Cell projection, dendritic spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • AUTS17 antibody
      • Cortactin binding protein 1 antibody
      • Cortactin SH3 domain-binding protein antibody
      • Cortactin-binding protein 1 antibody
      • CortBP1 antibody
      • CTTNBP1 antibody
      • GKAP/SAPAP interacting protein antibody
      • GKAP/SAPAP-interacting protein antibody
      • KIAA1022 antibody
      • Proline rich synapse associated protein 1 antibody
      • Proline-rich synapse-associated protein 1 antibody
      • ProSAP1 antibody
      • SH3 and multiple ankyrin repeat domains 2 antibody
      • SH3 and multiple ankyrin repeat domains protein 2 antibody
      • SHAN2_RAT antibody
      • SHANK antibody
      • Shank2 antibody
      • SPANK-3 antibody
      see all

    Images

    • Western blot detection of SHANK2 in Rat braim membrane lysates using ab94575 at 1:1000 dilution.

    References

    ab94575 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab94575.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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