Anti-SHANK3 antibody [N69/46] (ab93607)


  • Product name
    Anti-SHANK3 antibody [N69/46]
    See all SHANK3 primary antibodies
  • Description
    Mouse monoclonal [N69/46] to SHANK3
  • Host species
  • Specificity
    No cross-reactivity with SHANK1 or SHANK2.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 840 - 857 of Rat SHANK3 (Acc. No. Q9JLU4).

  • General notes

    The clone number has been updated from S69-46 to N69/46, both clone numbers name the same antibody clone.




Our Abpromise guarantee covers the use of ab93607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 190 kDa (predicted molecular weight: 186 kDa).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 0.1 - 1 µg/ml. Antigen retrieval is not essential but may optimise staining.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.



  • Function
    Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.
  • Tissue specificity
    Expressed in the cerebral cortex and the cerebellum.
  • Involvement in disease
    Note=A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B.
    Note=Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome).
    Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15) [MIM:613950]. SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
  • Sequence similarities
    Contains 6 ANK repeats.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 SH3 domain.
  • Cellular localization
    Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Postsynaptic density of neuronal cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI841104 antibody
    • DEL22q13.3 antibody
    • KIAA1650 antibody
    • Proline rich synapse associated protein 2 antibody
    • Proline-rich synapse-associated protein 2 antibody
    • ProSAP2 antibody
    • PSAP2 antibody
    • SH3 and multiple ankyrin repeat domains 3 antibody
    • SH3 and multiple ankyrin repeat domains protein 3 antibody
    • SH3/ankyrin domain gene 3 antibody
    • SHAN3_HUMAN antibody
    • Shank postsynaptic density protein antibody
    • Shank3 antibody
    • Shank3b antibody
    • SPANK 2 antibody
    • SPANK2 antibody
    see all


  • Anti-SHANK3 antibody [N69/46] (ab93607) at 1/1000 dilution + Rat brain membrane lysate at 15 µg

    Sheep Anti-Mouse IgG-HRP

    Predicted band size: 186 kDa

  • Immunohistochemical analysis of mouse backskin tissue sections labling SHANK3 with ab93607 at 1/100 dilution. FITC Goat anti-mouse at 1/50 was used as the secondary antibody. 


  • Overlay histogram showing SH-SY5Y cells stained with ab93607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


ab93607 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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I am happy to send this out as soon as possible. Can you confirm your previous order number for ab93607? I can't seem to locate it in our system and need either the order number or PO to arrange for the replacement ...

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I would like to suggest ab104702. There is a short lead time, but it is validated in Rat and WB. Please let me know if you would like to receive this antibody as a replacement.

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In searching for a protocol for ab80983, I have confirmed that this product was tested with the recombinant protein used as the immunogen, but not with endogenous protein. Therefore, I do not have an optimized ...

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I am sorry that you are having difficulties with this antibody in WB. Looking at your blot, it seems that the 1:500 (5ug/ml)dilution is producing a faint band at the predicted molecular weight and using a highe...

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I am sorry that you are experiencing difficulties with this antibody in WB. Below, please find the protocol used with this antibody.

1. Transfer and immobilize antigen on nitrocellulose or PVDF membr...

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Thank you for contacting us.

The product was purified from acites (ascitic fluid).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting Abcam. I have attached a WB image demonstrating the specificity of this antibody. It's a western in brain membrane lysates using a 1:1000 dilution. I hope this information is helpful. Please do not hesitate to contact us if you...

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