Overview

  • Product nameAnti-SHARP1 antibody
    See all SHARP1 primary antibodies
  • Description
    Rabbit polyclonal to SHARP1
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (Human) conjugated to KLH

  • Positive control
    • SH-SY5Y nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab82825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/35000 is determined for one of the three peptides.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be a transcriptional repressor that represses both basal and activated transcription.
    • Tissue specificityHighly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.
    • Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 Orange domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic helix-loop-helix domain containing class B3 antibody
      • BHE41_HUMAN antibody
      • bHLH protein DEC2 antibody
      • bHLHB3 antibody
      • bHLHe41 antibody
      • Class B basic helix loop helix protein 3 antibody
      • Class B basic helix-loop-helix protein 3 antibody
      • Class E basic helix-loop-helix protein 41 antibody
      • DEC2 antibody
      • Differentially expressed in chondrocytes protein 2 antibody
      • Enhancer of split and hairy related protein 1 antibody
      • Enhancer-of-split and hairy-related protein 1 antibody
      • hDEC2 antibody
      • Moderately similar to basic-helix loop helix protein M.musculus antibody
      • SHARP-1 antibody
      see all

    Anti-SHARP1 antibody images

    • Anti-SHARP1 antibody (ab82825) at 1/500 dilution + SH-SY5Y nuclear extract at 30 µg

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa
      Additional bands at : 209 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-SHARP1 antibody (ab82825)

    ab82825 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab82825.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"