Overview

  • Product name
  • Description
    Rabbit polyclonal to SHARP1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    3 synthetic peptides (Human) conjugated to KLH

  • Positive control
    • SH-SY5Y nuclear extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab82825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/35000 is determined for one of the three peptides.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      May be a transcriptional repressor that represses both basal and activated transcription.
    • Tissue specificity
      Highly expressed in skeletal muscle and brain, moderately expressed in pancreas and heart, weakly expressed in placenta, lung, liver and kidney.
    • Sequence similarities
      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 Orange domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Basic helix-loop-helix domain containing class B3 antibody
      • BHE41_HUMAN antibody
      • bHLH protein DEC2 antibody
      • bHLHB3 antibody
      • bHLHe41 antibody
      • Class B basic helix loop helix protein 3 antibody
      • Class B basic helix-loop-helix protein 3 antibody
      • Class E basic helix-loop-helix protein 41 antibody
      • DEC2 antibody
      • Differentially expressed in chondrocytes protein 2 antibody
      • Enhancer of split and hairy related protein 1 antibody
      • Enhancer-of-split and hairy-related protein 1 antibody
      • hDEC2 antibody
      • Moderately similar to basic-helix loop helix protein M.musculus antibody
      • SHARP-1 antibody
      see all

    Images

    • Anti-SHARP1 antibody (ab82825) at 1/500 dilution + SH-SY5Y nuclear extract at 30 µg

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 50 kDa
      Observed band size : 50 kDa
      Additional bands at : 209 kDa. We are unsure as to the identity of these extra bands.

    References

    ab82825 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82825.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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