Overview

  • Product nameAnti-SHARP2 antibody
    See all SHARP2 primary antibodies
  • Description
    Rabbit polyclonal to SHARP2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SHARP2.

    (Peptide available as ab25843.)

  • Positive control
    • This antibody gave a positive control in the following human lysate: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate This antibody gave a positive control in the following mouse lysate: Liver tissue This antibody gave a positive control in the following rat lysate: Liver tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab23797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 46 kDa).

Target

  • RelevanceSHARP2 (enhancer–of-split and hairy-related protein, differentially expressed in chondrocytes protein 1, DEC1) is a class B basic helix-loop-helix protein. Function: May function as a transcriptional factor for neuronal differentiation. PTM: Ubiquitinated; which may lead to proteasomal degradation
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • BHLHB2 antibody
    • bHLHe40 antibody
    • Class B Basic Helix Loop Helix Protein 2 antibody
    • Class E basic helix loop helix protein 40 antibody
    • Clast5 antibody
    • DEC1 antibody
    • Differentially expressed in chondrocytes protein 1 antibody
    • E47 interaction protein 1 antibody
    • EIP1 antibody
    • Enhancer of split and hairy related protein 2 antibody
    • SHARP 2 antibody
    • Stimulated by retinoic acid gene 13 protein antibody
    • STRA13 antibody
    see all

Anti-SHARP2 antibody images

  • All lanes : Anti-SHARP2 antibody (ab23797) at 1 µg

    Lane 1 : Hela Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size : 46 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa (possible non-specific binding),37 kDa (possible cleavage fragment,non-specific binding).
  • All lanes : Anti-SHARP2 antibody (ab23797) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate - normal tissue
    Lane 2 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 33 kDa,37 kDa. We are unsure as to the identity of these extra bands.

References for Anti-SHARP2 antibody (ab23797)

This product has been referenced in:
  • Vieira E  et al. RELATIONSHIP BETWEEN AMPK AND THE TRANSCRIPTIONAL BALANCE OF CLOCK-RELATED GENES IN SKELETAL MUSCLE. Am J Physiol Endocrinol Metab : (2008). Read more (PubMed: 18728219) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 16 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"