The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/5000 - 1/15000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).
Use at 1-4 µg/mg of lysate.
RelevanceSHARP2 (enhancer–of-split and hairy-related protein, differentially expressed in chondrocytes protein 1, DEC1) is a class B basic helix-loop-helix protein.
Function: May function as a transcriptional factor for neuronal differentiation.
PTM: Ubiquitinated; which may lead to proteasomal degradation
Detection of Human SHARP2 by Immunoprecipitation in whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded), using ab70723 at 3 µg/mg lysate (Lane 1). Lane 2 represents rabbit IgG IP control. Subsequent Western blot detection of SHARP2 was performed using ab70723 at 1 µg/ml. Chemiluminescence with exposure time of 30 seconds
References for Anti-SHARP2 antibody (ab70723)
has not yet been referenced specifically in any publications.
Publishing research using ab70723? Please let us know so that we can cite the reference in this datasheet.
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"