For the best experience on the Abcam website please upgrade to a modern browser such as
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Purity Protein A purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).
Function May have a role in normal immune development and control of inflammation.
Tissue specificity Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
Sequence similarities Contains 1 RanBP2-type zinc finger.
Cellular localization Cytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
Information by UniProt
Shank associated RH domain interacting protein antibody
Anti-SHARPIN antibody images
Western blot - SHARPIN antibody (ab69507)
All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilution Lane 1 : SHARPIN transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 25 µg per lane. Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution Predicted band size : 34 kDa Observed band size : 36 kDa ( why is the actual band size different from the predicted?)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"