Overview

  • Product nameAnti-SHARPIN antibody
    See all SHARPIN primary antibodies
  • Description
    Mouse polyclonal to SHARPIN
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human SHARPIN protein (AAH25244.1, 1 a.a. ~ 326 a.a)

  • Positive control
    • SHARPIN transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab69507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa. (predicted molecular weight: Isoform 2, 34 kDa; Isoform 1, 40 kDa).

Target

  • FunctionMay have a role in normal immune development and control of inflammation.
  • Tissue specificityHighly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similaritiesContains 1 RanBP2-type zinc finger.
  • Cellular localizationCytoplasm. Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp434N1923 antibody
    • hSIPL1 antibody
    • Shank associated RH domain interacting protein antibody
    • SHANK associated RH domain interactor antibody
    • Shank interacting protein like 1 antibody
    • Shank-associated RH domain-interacting protein antibody
    • Shank-interacting protein-like 1 antibody
    • Sharpin antibody
    • SHRPN_HUMAN antibody
    • SIPL1 antibody
    see all

Anti-SHARPIN antibody images

  • All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilution

    Lane 1 : SHARPIN transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

References for Anti-SHARPIN antibody (ab69507)

This product has been referenced in:
  • Verhelst K  et al. A20 inhibits LUBAC-mediated NF-?B activation by binding linear polyubiquitin chains via its zinc finger 7. EMBO J 31:3845-55 (2012). Read more (PubMed: 23032186) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"