Recombinant Anti-SHB antibody [EPR7976] (ab129190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7976] to SHB
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SHB antibody [EPR7976]
See all SHB primary antibodies -
Description
Rabbit monoclonal [EPR7976] to SHB -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SHB aa 450-550. The exact sequence is proprietary.
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Positive control
- HepG2 and K562 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.21 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7976 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa. |
Target
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Function
Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 SH2 domain. -
Domain
The SH2 domain preferentially binds phosphopeptides with the consensus sequence Y-[TVI]-X-L and mediates interaction with PDGFRA, PDGFRB, FGRFR1, IL2RB, IL2RG, CD3Z and CRK/CrKII. -
Post-translational
modificationsPhosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGFR1 or VEGFR2 activation. -
Cellular localization
Cytoplasm. Cell membrane. Associates with membrane lipid rafts upon TCR stimulation. - Information by UniProt
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Database links
- Entrez Gene: 6461 Human
- Entrez Gene: 230126 Mouse
- Entrez Gene: 362513 Rat
- Omim: 600314 Human
- SwissProt: Q15464 Human
- SwissProt: Q6PD21 Mouse
- Unigene: 521482 Human
- Unigene: 251716 Mouse
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Alternative names
- bA3J10.2 antibody
- OTTHUMP00000021397 antibody
- RP11 3J10.8 antibody
see all
Images
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All lanes : Anti-SHB antibody [EPR7976] (ab129190) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab129190 has been referenced in 1 publication.
- Vanli G et al. Evaluation and validation of commercial antibodies for the detection of Shb. PLoS One 12:e0188311 (2017). PubMed: 29194461