Overview

  • Product name
  • Description
    Rabbit polyclonal to SHC
  • Specificity
    ab24787 recognises SHC protein.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Immunogen

    Synthetic peptide (Human). Amino acid residues within the C-terminal region of human SHC.

  • Positive control
    • A431, human Jurkat , HeLa , and SKNSH cells. Also rat brain tissue and rabbit spleen fibroblasts.

Properties

Applications

Our Abpromise guarantee covers the use of ab24787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/2000. WB: 1/1000. Detects bands of approximately 46,52, and 66 kDa corresponding to the molecular weight of SHC variants (predicted molecular weight: 63 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
    • Tissue specificity
      Widely expressed. Expressed in neural stem cells but absent in mature neurons.
    • Sequence similarities
      Contains 1 PID domain.
      Contains 1 SH2 domain.
    • Domain
      In response to a variety of growth factors, isoform p46Shc and isoform p52Shc bind to phosphorylated Trk receptors through their phosphotyrosine binding (PID) and/or SH2 domains. The PID and SH2 domains bind to specific phosphorylated tyrosine residues in the Asn-Pro-Xaa-Tyr(P) motif of the Trk receptors. Isoform p46Shc and isoform p52Shc are in turn phosphorylated on three tyrosine residues within the extended proline-rich domain. These phosphotyrosines act as docking site for GRB2 and thereby are involved in Ras activation.
    • Post-translational
      modifications
      Phosphorylated by activated epidermal growth factor receptor. Phosphorylated in response to FLT4 and KIT signaling. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 by PRKCB upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity). Tyrosine phosphorylated in response to FLT3 signaling (By similarity). Tyrosine phosphorylated by activated PTK2B/PYK2 (By similarity). Tyrosine phosphorylated by ligand-activated ALK. Tyrosine phosphorylated by ligand-activated PDGFRB. Tyrosine phosphorylated by TEK/TIE2. May be tyrosine phosphorylated by activated PTK2/FAK1; tyrosine phosphorylation was seen in an astrocytoma biopsy, where PTK2/FAK1 kinase activity is high, but not in normal brain tissue. Isoform p52Shc dephosphorylation by PTPN2 may regulate interaction with GRB2.
    • Cellular localization
      Cytoplasm; Mitochondrion matrix. Localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization and Mitochondrion. In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ26504 antibody
      • p66 antibody
      • p66SHC antibody
      • SH2 domain protein C1 antibody
      • SHC (Src homology 2 domain containing) transforming protein 1 antibody
      • SHC 1 antibody
      • SHC A antibody
      • SHC adaptor protein 1 antibody
      • Shc antibody
      • SHC transforming protein 1 antibody
      • SHC transforming protein antibody
      • SHC-transforming protein 1 antibody
      • SHC-transforming protein 3 antibody
      • SHC-transforming protein A antibody
      • SHC1 antibody
      • SHC1_HUMAN antibody
      • SHCA antibody
      • Src homology 2 domain-containing-transforming protein C1 antibody
      see all

    Anti-SHC antibody images

    • All lanes : Anti-SHC antibody (ab24787) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 63 kDa
      Observed band size : 46,52,66 kDa (why is the actual band size different from the predicted?)

    References for Anti-SHC antibody (ab24787)

    This product has been referenced in:
    • Cyr-Depauw C  et al. Chordin-Like 1 Suppresses Bone Morphogenetic Protein 4-Induced Breast Cancer Cell Migration and Invasion. Mol Cell Biol 36:1509-25 (2016). Read more (PubMed: 26976638) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Loading amount
    100000 cells
    Specification
    Jurkat cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 06 2010

    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - other (Mitochondrial fraction)
    Loading amount
    25 µg
    Specification
    Mitochondrial fraction
    Treatment
    SHC-1 SiRNA in lanes 2-5, NT SiRNA in lane 6
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Infra red blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    Submitted Dec 07 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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