Overview

  • Product name
    Anti-SHC4 antibody - N-terminal
    See all SHC4 primary antibodies
  • Description
    Rabbit polyclonal to SHC4 - N-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SHC4 aa 17-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6S5L8

  • Positive control
    • Human melanoma tissue; HepG2 and Jurkat cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 69 kDa.

Target

  • Function
    Activates both Ras-dependent and Ras-independent migratory pathways in melanomas. Contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1.
  • Tissue specificity
    Only expressed in melanomas. Weakly expressed in normal melanocytes and benign nevi. Highly expressed at the transition from radial growth phase to vertical growth phase and metastatic melanomas, when tumor cells acquire migratory competence and invasive potential.
  • Sequence similarities
    Contains 1 PID domain.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Phosphorylated; the phosphorylation is enhanced by EGF. Phosphorylation at Tyr-424 is required for the interaction with GRB2.
  • Cellular localization
    Cell junction > synapse > postsynaptic cell membrane. Colocalized with MUSK at the neuromuscular junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • hShcD antibody
    • MGC34023 antibody
    • Rai like protein antibody
    • Rai-like protein antibody
    • RaLP antibody
    • SH2 domain protein C4 antibody
    • SHC (Src homology 2 domain containing) family member 4 antibody
    • SHC 4 antibody
    • SHC adaptor protein 4 antibody
    • SHC antibody
    • SHC family member 4 antibody
    • SHC transforming protein 4 antibody
    • SHC transforming protein D antibody
    • SHC-transforming protein 4 antibody
    • SHC-transforming protein D antibody
    • Shc4 antibody
    • SHC4_HUMAN antibody
    • SHCD antibody
    • Src homology 2 domain containing family member 4 antibody
    • Src homology 2 domain containing transforming protein C4 antibody
    • Src homology 2 domain-containing-transforming protein C4 antibody
    see all

Images

  • All lanes : Anti-SHC4 antibody - N-terminal (ab174908) at 1/100 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 69 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling SHC4 with ab174908 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab174908 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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