Overview

  • Product name
  • Description
    Rabbit polyclonal to SHCBP1L
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen, corresponding to amino acids 498-583 (FPGEYQAANL ALLTDDIIIK GVGKREEIMI TSEPSRDSFV VSKADNVKLM HLSLIQQGTV DGIVVVESGH MTLENCILKC EGTGVC) of Human SHCBP1L.

  • Positive control
    • Human testis tissue; Human plasma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Tissue specificity
    Highly expressed in the testis.
  • Sequence similarities
    Contains 4 PbH1 repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700012A16Rik antibody
    • C1orf14 antibody
    • GE36 antibody
    • RGD1305609 antibody
    • RP5-1133A12.2 antibody
    • SHC binding and spindle associated 1 like antibody
    • SHC SH2 domain-binding protein 1-like protein antibody
    • SHCBP1L antibody
    • SHP1L_HUMAN antibody
    see all

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane, cytoplasm and cell junctions. Recommended concentration of ab122310 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SHCBP1L with ab122310. Staining shows strong cytoplasmic positivity in cells in seminiferous ducts.
  • All lanes : Anti-SHCBP1L antibody (ab122310) at 1/250 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique

References

ab122310 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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