Overview

  • Product nameSheep Anti-Cow IgG1 H&L (HRP)
    See all IgG1 secondary antibodies
  • Description
    Sheep polyclonal Secondary Antibody to Cow IgG1 (HRP)
  • Target speciesCow
  • SpecificityBy immunoelectrophoresis and ELISA ab112754 reacts specifically with bovine IgG1. This antibody may cross react with IgG1 from other species.
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure subclass specificity. ab112754 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP).
  • Conjugation notesMolar enzyme/antibody protein ratio is 4:1.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab112754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. with colorimetric detection. Use at 1/1000 - 1/30000 with chemiluminescent detection.
ELISA 1/10000 - 1/100000. as primary antibody.
IHC-P 1/200 - 1/500.
ICC/IF 1/200 - 1/500.

References for Sheep Anti-Cow IgG1 H&L (HRP) (ab112754)

ab112754 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"