Sheep Anti-Cow IgM H&L (HRP) (ab112752)
Key features and details
- Sheep Anti-Cow IgM H&L (HRP)
- Conjugation: HRP
- Host species: Sheep
- Isotype: IgG
- Suitable for: WB, ELISA, IHC-P, ICC/IF
Overview
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Product name
Sheep Anti-Cow IgM H&L (HRP)
See all IgM secondary antibodies -
Host species
Sheep -
Target species
Cow -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. ab112752 was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to horseradish peroxidase (HRP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112752 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/30000. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution.
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ELISA |
1/10000 - 1/100000. as primary antibody.
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IHC-P |
1/200 - 1/500.
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ICC/IF |
1/200 - 1/500.
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Notes |
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WB
1/1000 - 1/30000. Colorimetric: Use 1/1000-1/10000 dilution; Chemiluminescent: Use 1/1000-1/30000 dilution. |
ELISA
1/10000 - 1/100000. as primary antibody. |
IHC-P
1/200 - 1/500. |
ICC/IF
1/200 - 1/500. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab112752 has been referenced in 2 publications.
- Qi T et al. Seroepidemiology of Neosporosis in Various Animals in the Qinghai-Tibetan Plateau. Front Vet Sci 9:953380 (2022). PubMed: 35928116
- Zhang J et al. Serological Analysis of IgG and IgM Antibodies against Anaplasma spp. in Various Animal Species of the Qinghai-Tibetan Plateau. Animals (Basel) 12:N/A (2022). PubMed: 36230463