Sheep F(ab) Anti-Guinea pig IgG H&L (HRP) (ab136791)

Overview

  • Product name
    Sheep F(ab) Anti-Guinea pig IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
    Sheep
  • Target species
    Guinea pig
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-Fr, IHC-P, ICC/IF, ELISAmore details
  • Immunogen

    Highly purified whole immunoglobulin from Guinea pig.

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Constituents: 49% Tris buffered saline, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Very stringent purification protocol.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    Do not store in plastic tubes (i.e. eppendorf) to avoid losing activity
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/100 - 1/1000. 4CN (4-chloro-1naphtol): 25mg in 49.5ml of 0.1M pH 7.4 PBS + 5µl/ml 100% ethanol. Filter on filter paper. Add 25µl of 30% H2O2. Incubate 10 min at room temperature.
IHC-Fr 1/20 - 1/100. DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
IHC-P 1/20 - 1/100. DAB (diamino-benzidine): 0.50mg/ml in a 0.1M pH7.4 TRIS/HCl buffer + 5µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness.
ICC/IF 1/20 - 1/100.
ELISA 1/500 - 1/5000. OPD (orthophenylene diamine): 0.4mg/ml in a 0.1M pH5 phosphate citrate buffer + 0.4µl/ml H2O2 (30% hydrogen peroxide solution). Incubation 10 minutes in darkness. Stop reaction with V/2 volume of 1M H2SO4. Reading at 492nm

References

ab136791 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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