Overview

  • Product nameSheep F(ab')2 Anti-Rabbit IgG H&L (Cy3 ®)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • Specificityab50503 binds all rabbit Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Immunogen

    Rabbit IgG

  • ConjugationCy3 ®. Ex: 552nm, Em: 565nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe F(ab')2 fragment of sheep anti-rabbit IgG is isolated from a pepsin digest of antiserum. Immunospecific methods of purification were used to remove essentially all sheep serum proteins, including immunoglobulins, that do not specifically bind to rabbit IgG.
  • Conjugation notesThe Cy3-antibody conjugate is extensively dialyzed to remove unbound Cy3.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.

References for Sheep F(ab')2 Anti-Rabbit IgG H&L (Cy3 ®) (ab50503)

This product has been referenced in:
  • Schaer CA  et al. Mechanisms of haptoglobin protection against hemoglobin peroxidation triggered endothelial damage. Cell Death Differ 20:1569-79 (2013). Read more (PubMed: 23995229) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"