Overview

  • Product name
    Sheep F(ab')2 Anti-Rabbit IgG H&L (Cy3 ®)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    ab50503 binds all rabbit Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Immunogen

    Rabbit IgG

  • Conjugation
    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The F(ab')2 fragment of sheep anti-rabbit IgG is isolated from a pepsin digest of antiserum. Immunospecific methods of purification were used to remove essentially all sheep serum proteins, including immunoglobulins, that do not specifically bind to rabbit IgG.
  • Conjugation notes
    The Cy3-antibody conjugate is extensively dialyzed to remove unbound Cy3.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.

References for Sheep F(ab')2 Anti-Rabbit IgG H&L (Cy3 ®) (ab50503)

This product has been referenced in:
  • Schaer CA  et al. Mechanisms of haptoglobin protection against hemoglobin peroxidation triggered endothelial damage. Cell Death Differ 20:1569-79 (2013). Read more (PubMed: 23995229) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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