Sheep Anti-Mouse IgG H&L (FITC) (ab6804)

Overview

  • Product nameSheep Anti-Mouse IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target speciesMouse
  • Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Mouse IgG whole molecule

  • ConjugationFITC. Ex: 493nm, Em: 528nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesDiaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notessuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • References for Sheep Anti-Mouse IgG H&L (FITC) (ab6804)

    ab6804 has not yet been referenced specifically in any publications.

    Product Wall

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    I am really not sure where these experiments may be going wrong, with so many different conditions tried I really cannot explain why no staining has been observed in every case.I...

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    I am sorry to hear that the new secondary antibody has not made a difference in the results obtained. I would be happy to try to help in understanding why this might be happening.I think it would be useful if...

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    I have arranged for the replacement to be sent to you. This is on the order number xxxxx(Purchase order number FOCR xxxxxx). This should be with you tomorrow. Please let me know if you have any problems r...

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    Thank you for confirming that.

    I can send you a new secondary antibody to try if you would like. I would suggest the following would be suitable:

    Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (FITC) (ab97239)
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    I am sorry but I am a little confused with what has been tested? Which alternative antibody was tried with the secondary antibody which again did not work? On the orderof the 16th of Jul...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"