Sheep Anti-Mouse IgG H&L (HRP) (ab102458)

Overview

  • Product nameSheep Anti-Mouse IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
  • Tested applicationsICC, ELISA, IHC-P, WBmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to HRP.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab102458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
ELISA 1/10000 - 1/100000. (Primary)
IHC-P 1/200 - 1/5000.
WB 1/10000 - 1/50000. with a chemoluminescence detection method. If using a colorimetric method, dilute 1/5000 - 1/30000.

References for Sheep Anti-Mouse IgG H&L (HRP) (ab102458)

ab102458 has not yet been referenced specifically in any publications.

Product Wall

Hi, glad to be of help, and Eve is REALLY glad you liked the brownies!

I have attached our dot blot protocol. However, on second thought, it may not be applicable to a blot of just the secondary, because I am not sure how well the HRP will handle the drying step that is at at the start of the attached protocol, after appl...

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Thank you for bringing this to our attention. It could very well be that the HRP is the issue, or the antibody:antibody affinity. I am assuming you are using the same primary with both secondaries (the more expensive one from another company and ours)....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"