Overview

  • Product name
    Sheep Anti-Mouse IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Target species
    Mouse
  • Tested applications
    Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, WB, ELISAmore details
  • Immunogen

    Mouse IgG whole molecule

  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.6 moles TRITC per mole of Rabbit IgG
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ICC/IF 1/1000 - 1/5000.
WB Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

References

ab6805 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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