Sheep Anti-Rabbit IgG H&L (ab97091)
Key features and details
- Sheep Anti-Rabbit IgG H&L
- Host species: Sheep
- Isotype: IgG
- Suitable for: ICC, IHC-P, WB, ELISA
Related conjugates and formulations
Overview
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Product name
Sheep Anti-Rabbit IgG H&L
See all IgG secondary antibodies -
Host species
Sheep -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, WB, ELISAmore details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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WB |
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000
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ELISA |
1/100 - 1/30000. Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
WB
1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000 |
ELISA
1/100 - 1/30000. Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab97091 has been referenced in 2 publications.
- Sun Q et al. microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway. Aging (Albany NY) 12:11446-11465 (2020). PubMed: 32544883
- Yan R et al. Downregulation of microRNA-4295 enhances cisplatin-induced gastric cancer cell apoptosis through the EGFR/PI3K/Akt signaling pathway by targeting LRIG1. Int J Oncol 53:2566-2578 (2018). PubMed: 30320337