Sheep Anti-Rabbit IgG H&L (DyLight® 650) (ab96926)
Key features and details
- Sheep Anti-Rabbit IgG H&L (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Sheep
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
Overview
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Product name
Sheep Anti-Rabbit IgG H&L (DyLight® 650)
See all IgG secondary antibodies -
Host species
Sheep -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96926 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/50 - 1/200.
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
Notes |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab96926 has been referenced in 2 publications.
- Stewart CM et al. Ebola virus triggers receptor tyrosine kinase-dependent signaling to promote the delivery of viral particles to entry-conducive intracellular compartments. PLoS Pathog 17:e1009275 (2021). PubMed: 33513206
- Bergsbaken T & Bevan MJ Proinflammatory microenvironments within the intestine regulate the differentiation of tissue-resident CD8? T cells responding to infection. Nat Immunol 16:406-14 (2015). IHC . PubMed: 25706747