By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200.
1/50 - 1/500.
1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
Emission spectra of DyLight® fluorochromes available in our catalog. Line colors represent the approximate visible colors of the wavelength maxima.
References for Sheep Anti-Rabbit IgG H&L (DyLight® 650) (ab96926)
This product has been referenced in:
Bergsbaken T & Bevan MJ Proinflammatory microenvironments within the intestine regulate the differentiation of tissue-resident CD8? T cells responding to infection. Nat Immunol16:406-14 (2015).
Read more (PubMed: 25706747) »