Overview

  • Product name
    Sheep Anti-Rat IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target species
    Rat
  • Tested applications
    Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • Conjugation
    Biotin

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
  • Conjugation notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality
    Polyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Immunomicroscopy
Dot blot
ICC/IF
IHC-P
IHC-Fr
  • Application notes
    immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
  • Images

    • Ab6851 was used at dilution 1/100 with the primary antibody ab56299 in IHC-P. See the review on ab56299.

    References

    This product has been referenced in:
    • Heuslein JL  et al. Mechanisms of Amplified Arteriogenesis in Collateral Artery Segments Exposed to Reversed Flow Direction. Arterioscler Thromb Vasc Biol 35:2354-65 (2015). Read more (PubMed: 26338297) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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