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Product name Sheep Anti-Rat IgG H&L (Biotin) See all IgG secondary antibodies
Target species Rat
Tested applications Suitable for:
WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr more details
Rat IgG whole molecule
Conjugation Biotin Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
Concentration information loading...
Purity IgG fraction
Purification notes Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC).
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
Conjugation notes This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
Sheep Anti-Rat IgG H&L (Biotin) images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Sheep polyclonal Secondary Antibody to Rat IgG - H&L (Biotin) (ab6851)
Ab6851 was used at dilution 1/100 with the primary antibody ab56299 in IHC-P. See the review on ab56299.
References for Sheep Anti-Rat IgG H&L (Biotin) (ab6851)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"